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Malaria protocols:

  • How to diagnose malaria?
  • 1.microscopy of blood film.
The malaria parasite density can be estimated by looking at a monolayer of red blood cells (RBCs) on the thin smear using the oil immersion objective at  100x.
The slide should be examined where the RBCs are more or less touching  (approximately 400 RBCs per field). The parasite density can then be estimated from the percentage of infected RBCs, after counting 500 to 2000 RBCs.

2. antigen detection tests (rapid diagnostic tests or RDTs) using a “dipstick”

3.QBC- Quantitative Buffy Coat (QBC) Test :  method for identifying the malarial parasite in the peripheral blood.

  • Malaria is a nationally notifiable disease and all cases should be reported to your  district or 
    state health department, 
  • categorization: 
    Patients diagnosed with malaria are generally 
    categorized as having either uncomplicated or severe malaria.

  • TREATMENT:                 chloroquin sensitive and resistant types.
    • A chloroquine dose of 600 mg base (= 1,000 mg salt)initially 
      • , followed by 300 mg base (= 500 mg salt) at 6, 24, and 48 hours after the 
      • initial dose for a total chloroquine dose of 1,500 mg base (=2,500 mg salt). 
  •                               for chloq resistant cases: 
  •  atovaquone proguanil (Malarone) or 
  • artemether-lumefantrine (Coartem). 
    •  Quinine sulfate plus doxycycline for 7days. 
    • infections with P. vivax and P. ovale 
        • can relapse due to hypnozoites that remain dormant in the liver. To eradicate the 
        • hypnozoites, patients should be treated with a 14-day course of primaquine 
        • phosphate


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